The CRISPR/Cas9 system includes the Cas9 nuclease, which can be programmed to target and cleave specific sequences in the genome through the use of a guide RNA (gRNA) that directs it to any user specified position in the genome. The Cas9 protein binds to the site specified by the gRNA and produces a targeted double-strand break in the DNA.This break is then repaired by the cell and can result in deletions and/or insertions resulting in gene inactivation. Alternatively, if a homologous template is also introduced, repair can occur via homologous directed repair resulting in precise, user-defined gene editing. This allows investigators to produce mutant cells by modifying a single or many nucleotides in a defined fashion.


Genome Engineering

iPSC Technology