Custom Cell Line Form

    Your Name (required)

    Your Email (required)

    Telephone

    Your Organization

    Project Objective

    Gene KnockoutTargeted DeletionCodon ChangeTargeted IntegrationPoint MutationGene TaggingOther

    Nuclease Preference?

    CRISPR

    Target Gene

    Species:
    HumanMouseRatOther

    Target Gene Name:

    Target Gene ID:

    Cell Line

    Cell Line of Choice:




    Alternate Cell Line:




    Are there any splice variants of your gene in your chosen cell line? YesNo

    If Yes, please provide mRNA ascession # of the isoform you wish to target:


    Are there any known SNPs in your gene in your chosen line? YesNo

    If Yes, please provide details:


    How many alleles do you wish to modify:

    AllOneOther

    Do you routinely use the chosen cell line?

    YesNo

    Any special growth requirement or characteristics?

    YesNo

    If Yes, please explain:


    Additional Information

    Does a transgenic model exist for this modification?

    YesNo

    Is the target highly expressed?

    YesNo

    Can the gene be knocked down?

    YesNo

    If Yes, did the knockdown affect cell growth?

    YesNo

    Do you suspect that the modification will affect cell growth or viability?

    YesNoExplain

    Please provide the sequence to be modified (as cDNA +/- 200bp) with specified mutation(s) marked in {brackets}.